Tips & Recommendations for PCR Beginners

We asked the science community on Twitter to share their favorite tips for PCR beginners:

“When using strips, BE SURE to label one end of the strip to know your reaction order. It’s easy to mix up which end you started on.”

@IHStreet

 

“Patience, patience, patience…And yeah, always controls. No exceptions.”

@GoldenMole

 

“Don’t forget to write down which well has your negative control. Also, PCR is witchcraft.”

@SFriedScientist

 

“Don’t mix flat and dome caps in the same cycle!”

@IHStreet

 

“Always run a control, run your samples in duplicate, use a gradient PCR machine, and take detailed notes.”

@HeidiKayDeidi

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