Tips & Recommendations for PCR Beginners

We asked the science community on Twitter to share their favorite tips for PCR beginners:

“When using strips, BE SURE to label one end of the strip to know your reaction order. It’s easy to mix up which end you started on.”



“Patience, patience, patience…And yeah, always controls. No exceptions.”



“Don’t forget to write down which well has your negative control. Also, PCR is witchcraft.”



“Don’t mix flat and dome caps in the same cycle!”



“Always run a control, run your samples in duplicate, use a gradient PCR machine, and take detailed notes.”


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